PATENTS
Detection Of Cardiac Troponin Or Biological Markers Via Shear Horizontal Surface Acoustic Wave Biosensor Using A Wet-Dry Bioanalytical Technique
Patent Number: US 2020/0309737 Al
Date Filed: October 1, 2020
Country Filed In: USA
Product Application: SAW Biosensor
Description:
The illustrated embodiments include a method of operating a SAW sensor to detect a sample in a fluid which includes the steps of providing a SAW sensor with a functionalized detection lane in a handheld, portable assay, and sensor system; maintaining the functionalized detection lane of the SAW sensor dry until the sample is fluidicly disposed of in the detection lane; fluidicly disposing of the sample in the functionalized detection lane; removing fluid the functionalized detection lane to concentrate the sample in the functionalized detection lane to increase the probability of a specific antibody-antigen interaction; washing the functionalized detection lane so that substantially only the specific antigenantibody interaction remains in the functionalized detection lane; removing fluid from the functionalized detection lane again, and measuring the concentration of the sample while the functionalized detection lane is fluid-free.
Field of Use: A surface acoustic wave (SAW) performs a rapid, label-free detection of biological species. Biosensing and detection of multiple analytes multiplexed by an array of sensing lanes are configured to enable bio-amplification using engineered DNA encoded libraries as the probe through a phage display procedure to enhance specificity, capture statistics for the detection, screening and analysis of the analyte in vitro. A biochemical formulation minimizes the limit of detection (LOD) at a threshold magnitude on the order of a femtomolar concentration. Additional enhancement of the apparatus is achieved by the use of an analog front end to amplify biochemical events.