PATENTS
An Apparatus For Automatic Sampling Of Biological Species Employing Disk Microfluidics System
Patent Number: US 2019/0285092 A1
Date Filed: July 4, 2019
Country Filed In: USA
Product Application: OPTIKUS Pathogen Detection
Description:
A field-portable diagnostic apparatus uses a rotatable disk in which a microfluidic circuit is defined. The microfluidic circuit includes a centrifugal separation chamber receiving a sample to stratify the sample. A magnetic bead holding chamber is communicated to a mixing chamber, where mass amplifying functionalized magnetic-nanoparticles, held in a buffer solution and contained in the magnetic bead holding reservoir communicated to mixing chamber, are mixed with the separated fluid delivered to the mixing chamber from the separation chamber. The functionalized magnetic nanoparticles conjugate with a target analyte in the sample. A magnet in proximity to a SAW chamber including a SAW detector draws the functionalized magnetic nanoparticles toward antibodies immobilized on the SAW sensor surface A wash reservoir is communicated to the SAW sensor chamber, and a cleanup/waste reservoir is communicated to the SAW chamber for receive fluid after it has passed through the SAW chamber.
Field of Use: OPTIKUS is the second generation of portable handheld pathogen detection technologies created by Josh Shachar and his Engineering Team. The device utilized a whole new method of taking a fluid sample and processing it through all steps of biomarker detection on a single microfluidic disc. Once a sample was injected into a septum reservoir on the disc, the disc was inserted into the OPTIKUS Reader. The microfluidic disc allows for fluid separation, conjugation of magnetic nano-beads to the suspected antigens, conjugation of the nano-bead-antigen particles to corresponding antibodies embedded on a biosensor surface, and then detection to occur via surface acoustic wave generation, all within minutes and with clinically relevant results. The reader was also capable of communicating the data through a secure wireless protocol to cloud storage for immediate dissemination and review by applicable healthcare provides.