PATENTS
Method For Isothermal DNA Detection Using A Modified CRISPR/CAS System And The Apparatus For Detection By Surface Acoustic Waves For Gene Editing
Patent Number: US 2017/597,090
Date Filed: July 27, 2017
Country Filed In: USA
Product Application: SAW Biosensor
Description:
A method of reducing the limit of detection in a surface acoustic wave sensor (SAW) includes the steps of attaching a plurality of DNA segments to a detection surface of a SAW; performing a CRISPR/Cas9 preparation of the DNA segments to cut and splice a selected protein into at least one of a plurality of the DNA segments; conjugating a nanoparticle to the selected protein, and measuring the number of DNA segments with conjugated nanoparticles using a surface acoustic wave sensor (SAW). The nanoparticle may be modified to form a single-electron transistor (SET) which generates a detectable signal in response to RF or ultrasonic excitation which is indicative of binding of the corresponding nanoparticle to a selected target analyte.
Field of Use: Field of Use: The SAW Biosensor is part of Josh Shachar's exploration into developing next-generation biosensors that are capable of detecting specific biomarkers within a sample at clinically relevant levels in a simple portable point-of-care-device that can provide physicians and healthcare workers rapid and affordable pathogen detection without the need for a dedicated lab and specialized technicians and all the associated cost therein. The SAW Biosensor technology represented a major leap forward in being able to lower the limits-of-detection and the time-to-detection of a biomarker by utilizing the generation of a Surface Acoustic Wave across a sensor-surface where targeted antigens had been captured from a sample of interest.